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Gibberellin-induced mesocotyl elongation in deep-sowing tolerant maize inbred line 3681-4

机译:赤霉素诱导的玉米深系自交系3681-4的胚轴伸长

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摘要

In order to elucidate the molecular mechanism of gibberellin (GA)-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combining Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying GA promotion of maize mesocotyl elongation. These studies showed that a mount of genes, such as the GA receptor GID1, the transcriptional factor MYB, and the genes encoding the DELLA protein DWRF8, kinases, Raf, LRR and RLCK, might play a critical role in maize mesocotyl elongation. Moreover, the genes that were involved in flavonoid biosynthesis, aminosugars metabolism, cell wall synthesis and modification were also found to be responsible for this process.
机译:为了阐明赤霉素(GA)诱导的胚轴伸长的分子机制,在耐深播玉米自交系3681-4中进行了基因表达谱分析。结合Affymetrix基因芯片分析和实时PCR的基因表达研究来确定GA促进玉米中胚轴延伸的分子机制。这些研究表明,大量基因,例如GA受体GID1,转录因子MYB,以及编码DELLA蛋白DWRF8,激酶,Raf,LRR和RLCK的基因,可能在玉米中胚轴伸长中起关键作用。此外,还发现了参与类黄酮生物合成,氨基糖代谢,细胞壁合成和修饰的基因。

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