Induced polyploidy with gigas expression for root traits in Capsicum annuum (L.)

摘要

P>Polyploidy was induced in hot pepper (Capsicum annuum cv. 'GVC-111') using colchicine and tetraploid plants with robust root size system were obtained. Chromosome doubling was achieved by different concentrations of colchicine (0.05%, 0.1%, 0.2% and 0.4% aqueous solution) to either presoaked seeds or to the shoot tip of young seedlings for 24, 48 and 72 h, respectively. A total of 313 plants with significantly larger average stomata length (48.6%), lower frequency of stomata per square mm (41.7%) and a higher number of chloroplast in guard cells (47.3%) were selected as putative polyploids for further analysis by flow cytometry. Out of 313 putative polyploids, 31 were tetraploid, 270 were mixoploid and 12 were, in fact, diploid. Six tetraploid plants were selected for robust root system observations, longer primary root length and higher density of lateral roots. This study demonstrates successful utilization of colchiploidy to create novel mutations for root size systems.