Genetic diversity and population structure of the selected core set in Amaranthus using SSR markers.

摘要

The genus Amaranthus has gained much attention, particularly for its high economic and nutritional value. It is a genus of taxonomic complexity with many interspecific hybrids. For effective conservation and management of the germplasm collected, the development of a core set of accessions is especially important. A core set of 63 accessions was successfully developed from an entire collection of 634 accessions using the POWERCORE 1.0. Among the tested methods for developing the core set - the advanced M strategy using the modified heuristic method (HCC), randomly chosen collections and stratified random collections - HCC was found to be best, with 100% coverage of alleles and minimum redundancy. Allele frequency distribution of the core set developed with HCC was highly correlated with that of entire collections (r=0.91). Model-based structure analysis revealed the presence of three subpopulations and 11 admixtures in the selected core set, which is basically consistent with clustering based on the genetic distance. The results from this study will provide effective information for future germplasm conservation and improvement programmes in Amaranthus.Digital Object Identifier http://dx.doi.org/10.1111/pbr.12027