Development of a cytoplasmic male-sterile line of eggplant (Solanum melongena L.) with the cytoplasm of Solanum anguivi.

摘要

To develop a new cytoplasmic male-sterile line of eggplant utilizing the Solanum anguivi cytoplasm, the interspecific hybrid (S. anguivi x eggplant 'Senryo 2 gou') was repeatedly backcrossed to eggplant 'Uttara' up to BC₅ generation to achieve cytoplasm substitution. Chloroplast and mitochondrial DNA analyses confirmed that backcross progenies had the S. anguivi cytoplasm. In the backcross progenies, male-sterile plants, whose anthers were completely devoid of pollen grains, appeared. All the backcross generations obtained from the male-fertile seed parents segregated into male-fertile and sterile types with a 3:1 ratio, whereas those obtained from the male-sterile one were all male-sterile. The selfed progeny from a male-fertile BC₅ plant (selfed progeny 1) and the selfed progeny from the male-fertile selfed progeny 1 segregated at 15:1 and 3:1 ratios, respectively. These results indicated that two independent dominant fertility restorer (Rf) genes control pollen formation. A new male-sterile line of eggplant could be developed by utilizing the cytoplasm of S. anguivi.