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Expression profiling identifies differentially expressed genes associated with the fusarium head blight resistance QTL 2DL from the wheat variety Wuhan-1

机译:表达谱分析鉴定了与小麦品种武汉-1的枯萎病抗性QTL 2DL相关的差异表达基因

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To better understand the contribution of the quantitative fusarium head blight (FHB) resistance locus, 2DL QTL from Wuhan-1, we have characterized two pairs of sister lines carrying either the 2DL QTL or no known QTLs. In wheat spikes, our visual and microscopic observations suggested that the 2DL QTL suppress fungal infection in inoculated spikelets and reduce spread from spikelets to rachis. Transcriptome profiling of infected spikelets showed changes in expression for 487 probe sets between the 2DL-carrying and the null lines. Of 27 transcripts selected for validation, eight candidate genes with similar expression profiles between the two pair of lines were identified. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.
机译:为了更好地了解武汉-1镰刀菌疫病抗性定量位点2DL QTL的贡献,我们鉴定了两对携带2DL QTL或未知QTL的姊妹系。在小麦穗状花序中,我们的视觉和显微镜观察表明2DL QTL抑制了接种的穗状花序中的真菌感染,并减少了从穗状花序到小穗的传播。感染小穗的转录组图谱分析显示,载有2DL肽和零线之间的487个探针组的表达发生了变化。在选择用于验证的27个转录本中,鉴定了两对品系之间具有相似表达谱的八个候选基因。 Crown版权所有(C)2015,由Elsevier Ltd.发行。保留所有权利。

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