首页> 外文期刊>Journal of Clinical Microbiology >Verification of causal relationships between Listeria monocytogenes isolates implicated in food-borne outbreaks of listeriosis by randomly amplified polymorphic DNA patterns.
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Verification of causal relationships between Listeria monocytogenes isolates implicated in food-borne outbreaks of listeriosis by randomly amplified polymorphic DNA patterns.

机译:通过随机扩增的多态性DNA模式验证单核细胞增生李斯特菌分离株之间的因果关系,涉及食品传播的李斯特菌病暴发。

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Food and clinical isolates of Listeria monocytogenes recovered from four different outbreaks of listeriosis were analyzed by their PCR-based randomly amplified polymorphic DNA (RAPD) patterns to verify their causal relationships. The generation of DNA fingerprints by PCR-based RAPD analysis is a fast and sensitive method for the epidemiological tracking and identification of bacteria implicated in food poisoning outbreaks. The L. monocytogenes strains used in the study were obtained from the following four outbreaks: California, 1985, Mexican-style cheese; Canadian Maritime Provinces, 1981, coleslaw; Canada, 1989, brie cheese; and Canada, 1989, alfalfa tablets. RAPD profiles were generated by using random 10-mer primers for at least one food and one clinical isolate recovered from each outbreak. Identical profiles for 20 different primers were observed for each pair of food and clinical isolates from two of the four outbreaks. Isolates from the outbreak involving alfalfa tablets exhibited identical patterns for 19 primers; however, primer OPA-1 produced one additional 1.8-kb fragment, designated OPA-1-1.8, that was found in the food isolate but not in the corresponding clinical isolate. Hybridization analysis revealed that the absence of the OPA-1-1.8 polymorphic fragment in the clinical isolate was due to a deletion of at least 1.8 kb. Loss of the OPA-1-1.8 polymorphic fragment could not be induced by infective passage of the L. monocytogenes isolate from the alfalfa tablet through a mouse or by growth of this isolate under selective conditions. This suggests that the isolate recovered from the food was not identical to the isolate recovered from the patient. The ability to produce unique RAPD patterns allows for the discrimination between isolates even if they are of the same serotype and multilocus enzyme electrophoretic type.
机译:通过基于PCR的随机扩增多态性DNA(RAPD)模式分析了从四个不同的李斯特菌病暴发中回收的李斯特菌的食物和临床分离株,以验证其因果关系。通过基于PCR的RAPD分析生成DNA指纹是一种快速,灵敏的方法,用于流行病学跟踪和识别与食物中毒暴发有关的细菌。本研究中使用的单核细胞增生李斯特氏菌菌株是从以下四次暴发中获得的:加利福尼亚州,1985年,墨西哥风味干酪;加拿大海事省,1981年,凉拌卷心菜;加拿大,1989年,干酪奶酪;和加拿大,1989年,苜蓿片。通过使用随机的10-mer引物针对至少一种食物和从每次暴发中回收的一种临床分离株生成RAPD图。对于四个暴发中的两次暴发中的每对食物和临床分离株,观察到了20种不同引物的相同图谱。涉及苜蓿片的暴发分离株对19种引物表现出相同的模式。但是,引物OPA-1产生了另一个1.8kb的片段,称为OPA-1-1.8,该片段在食品分离株中发现,但在相应的临床分离株中未发现。杂交分析显示临床分离物中不存在OPA-1-1.8多态性片段是由于至少1.8 kb的缺失所致。 OPA-1-1.8多态性片段的丢失不能通过紫花苜蓿片中单核细胞增生李斯特氏菌的分离株通过小鼠或在选择性条件下的生长来诱导。这表明从食物中回收的分离物与从患者身上回收的分离物不同。产生独特的RAPD模式的能力可以区分分离株,即使它们具有相同的血清型和多位点酶电泳类型。

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