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Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)

机译:微小君子兰和君子兰(Amaryllidaceae)中14种微卫星标记的分离和鉴定

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Clivia is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild Clivia species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of C. miniata Regel. and Clivia nobilis Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 Clivia accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of Clivia species and cultivars.
机译:君子兰是非常重要的园艺植物,在世界各地已被广泛用作观赏植物。为了评估野生君子兰物种和栽培品种的亲缘关系和遗传多样性,我们使用FIASCO从单个小隐孢子虫的单个克隆中分离了富含AC的重复序列。还有君子兰(Clivia nobilis Lindl)。在这14个重复中,有10个是多态的,有4个是单态的。多态性标记基因座使用61个君子兰登录号进行了表征。等位基因的数目范围为2至6,观察到的杂合度为0.04至1.00,预期杂合度为0.04至0.83。这些微卫星标记基因座为将来研究君子兰的品种和品种提供了工具。

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