TRIGONELLINE AND SITAGLIPTIN ATTENUATES NICOTINAMIDE-STREPTOZOTOCIN INDUCED DIABETIC NEPHROPATHY IN WISTAR RATS

摘要

Objective: The objective of present study was to evaluate the effect of trigonelline (TRIG), sitagliptin (SITA) alone and concomitant administration in nicotinamide-streptozotocin induced diabetic nephropathy in Wistar rats. Methods: Diabetes was induced by streptozotocin (65 mg/kg i.p.) injected 15 min after nicotinamide (110 mg/kg, i.p.). Four week later rats were randomly selected and divided into five groups (n=6). The rats were divided into following groups. Group I: non-diabetic. Group II: diabetic control, Group III: diabetic + TRIG (50 mg/kg), Group IV: diabetic + SITA (5 mg/kg), Group V: diabetic + [TRIG (50 mg/kg) + SITA (5 mg/kg)].The drugs were administered for next 4 weeks starting from 5thweek post nicotinamide-STZ injection. Serum glucose (SG) and body weight were measured weekly. Serum creatinine, blood urea nitrogen, serum uric acid, urine volume and creatinine were measured at 0, 4thand 8thweek of the study. While superoxide dismutase, reduced glutathione, malondialdehyde and kidney weight measurement and histopathological were carried out at the end of study period. Results: Concomitant administration of TRIG + SITA showed significant decrease in SG, kidney weight, serum creatinine, blood urea nitrogen, serum uric acid, urine volume and the level of malondialdehyde. While significant increase in urine creatinine, activity of superoxide dismutase and glutathione were observed compared to effect of either drug alone. Histopathological study confirmed that TRIG + SITA prevented structural kidney damage. Conclusion: Concomitant administration of trigonelline with sitagliptin prevented development of diabetic nephropathy in rats