ISOLATION AND CHARACTERIZATION OF β-SITOSTEROL FROM METHANOLIC EXTRACT OF CORDIA DICHOTOMA LINN. BARK

摘要

emCordia dichotoma/em Linn. belonging the family Boraginaceae is locally called as Lasora in India. It is an important drug to cure diseases of the kidney, liver, spleen, heart and blood which attribute many medicinal properties in Ayurveda. The purpose of this study is to identify and characterize the bioactive principle from the bark of emCordia dichotoma/em Linn. Phytochemical screening methanolic extract of bark of emCordia dichotoma/em Linn. was done and showed the presence of phytosterol. Phytosterols were isolated from the methanol extract by admixing 100 g of extract with 200 ml of acetonitrile. The resulting white sterol crystals were analyzed by Thin Layer Chromatography and High Performance Liquid Chromatography (HPLC) for the purification. The sterol for structure elucidation was further studied by more sophisticated analytical techniques eme.g/em. FTIR, sup1/supH-NMR, sup13/supC-NMR and LC-MS. After various spectral analysis and interpretation of same, the isolated compound was identified as emβ-/emsitosterol.