Effects of vitamin C polyphosphate on antioxidase gene transcription activity in mouse liver

摘要

The study was conducted to investigate the effects of vitamin C polyphosphate on transcription activity of antioxidase gene in mice liver. 24 male mice (3–4 weeks old) with an initial body weight of 16–22 g were randomly divided into four groups. These were fed with 35% vitamin C polyphosphate supplementation at the dosages of 0, 500, 2 500 and 5 000 mg/kg of the diet, respectively for 4 weeks. In order to evaluate transcription activity of liver antioxidase gene, livers were collected to detect the mRNA of superoxide dismutases (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) by RT-PCR . Results showed that vitamin C polyphosphate had significant effects on transcription activity of antioxidase gene in mouseliver. The CAT mRNA in the groups supplemented with vitamin C polyphosphate at 2 500 and 5 000 mg/ kg were significantly higher than that of control group. The SOD mRNA in the groups supplemented with vitamin C polyphosphate at 2 500 mg/kg were higher than that of the control group, and the 5 000 mg/kg group were higher than those of other three groups. The GSH-Px mRNA in the groups supplemented with vitamin C polyphosphate at 5 000 mg/kg was notably higher than those of other three groups. These results suggest that vitamin C polyphosphate can improve the transcription activity of the antioxidase gene in mouse liver, while the requirement for vitamin C polyphosphate to improve transcription activities of the antioxidase gene varies [ Acta Zoologica Sinica 52(6):1107– 1112, 2006] .