A comparative study on in vitro antioxidant activity of the legumes Acacia auriculiformis and Acacia ferruginea with a conventional legume Cajanus cajan Estudio comparativo de la actividad antioxidante in vitro de las legumbres Acacia auriculif

摘要

The total phenolic content and antioxidant activities of Acacia auriculiformis, Acacia ferruginea and Cajanus cajan seed extracts were examined. The investigation revealed that raw and processed seed extracts had substantial concentration of dietary phenolics including tannins and high molecular condensed tannins; however, no condensed tannins were detected in A. auriculiformis . The antioxidant activity of extracts were assessed by using different in vitro models such as reducing power, hydroxyl radical (OH ?￠???￠ ), 2,2?￠????diphenyl-1-picryl-hydrazyl (DPPH ?￠???￠ ), 2,2?￠???2azinobis(3-ethylbenzothiozoline-6-sulfonic acid) diammonium salt (ABTS ?￠???￠+ ), linoleic acid emulsion system, metal chelation and antihemolytic activity. All the extracts showed dose dependent activity in reducing power, hydroxyl radical and DPPH ?￠???￠ radical scavenging activities. Interestingly, the maximum level of peroxidation inhibiting activity (91.3%?￠????94.1%) was observed at the concentration of 1????mg in the final reaction mixture. Further, all the sample extracts exhibited potential metal chelating and ABTS ?￠???￠+ cation radical scavenging activities. At 500????????g of extract in the reaction mixture, the antihemolytic activity was found to be moderate. Se analiz???3 el contenido total fen???3lico y actividades antioxidantes de extractos de semillas de Acacia auriculiformis, Acacia ferruginea y Cajanus cajan . La investigaci???3n revel???3 que extractos de semillas crudas y procesadas pose???-an unaconcentraci???3n sustancial de fen???3licos alimenticios, incluyendo taninos y taninos condensados de alto peso molecular; sin embargo, no se detectaron taninos condensados en A. auriculiformis . La actividad antioxidante de los extractos fue calificada usando diferentes modelos in vitro , tales como poder reductor, OH ?￠???￠ , DPPH ?￠???￠ , ABTS ?￠???￠+ , sistema emulsi???3n ????cido linoleico, quelaci???3n de metal y actividad antihemol???-tica. Dependiendo de la dosis, todos los extractos mostraron actividad en poder reductor, radical hidroxilo y actividades de captaci???3n de radical DPPH ?￠???￠ . Curiosamente, el nivel m????ximo de actividad inhibidora de peroxidaci???3n (91.3%?￠????94.1%) se observ???3 en la concentraci???3n de 1????mg en la mezcla de reacci???3n final. Adem????s, todos las muestras de extractos presentaron potencial como quelante met????licos y actividades de captaci???3n del radical cati???3n ABTS ?￠???￠+ . Con 500????????g de extracto en la mezcla reacci???3n, la actividad antihemol???-tica result???3 ser moderada.