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Current Protocols in Protein Science

中文名称：当前协议在蛋白质科学

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1.GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip

机译：GlycoBIST:系统自动多糖配置目标蛋白质使用Milli-Bead数组提示
- 作者：Hiroko Shimazaki;Ayaka Ono;Masako Tsuruga
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
2.Magnetic Resonance Relaxometry for Determination of Protein Concentration and Aggregation

机译：磁共振王仁贵的决心蛋白质的浓度和聚合
- 作者：Marc B. Taraban;Katharine T. Briggs;Yihua Bruce Yu
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
3.Purification of Ciliary Tubulin from Chlamydomonas reinhardtii

机译：从衣藻净化纤毛的微管蛋白reinhardtii
- 作者：Ron Orbach;Jonathon Howard
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
4.Two-Step Preparation of Highly Pure, Soluble HIV Protease from Inclusion Bodies Recombinantly Expressed in Escherichia coli

机译：两步制备高度纯,可溶性艾滋病毒蛋白酶从包涵体的重组大肠杆菌中表达
- 作者：Dean Sherry;Roland Worth;Yasien Sayed
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
5.Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis

机译：评估的高阶结构制定由2 d单克隆抗体疗法甲基核磁共振相关和主成分分析
- 作者：Luke W. Arbogast;Frank Delaglio;Robert G. Brinson
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
- therapeutic protein;
- higher-order;
- SpectraMonoclonal Antibodiesorder structure;
6.Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations

机译：制备重组膜蛋白毕赤酵母属pastoris分子调查
- 作者：Lucile Guyot;Lucie Hartmann;Sarah Mohammed-Bouteben
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
7.A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins

机译：快速和灵巧的净化方法Glycan-Binding蛋白和糖蛋白
- 作者：Christina J. Welch;Priyanka D. Kadav;Jared L. Edwards
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
8.Synthesis of Recombinant Human Hemoglobin With NH2-Terminal Acetylation in Escherichia coli

机译：重组体人血红蛋白的合成在大肠杆菌NH2-Terminal乙酰化作用
- 作者：Chandrasekhar Natarajan;Anthony V. Signore;Vikas Kumar
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
9.A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time

机译：监控SUMOylation荧光技术分析在实时
- 作者：Vasvi Tripathi;Ranabir Das
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
10.PLiMAP: Proximity-Based Labeling of Membrane-Associated Proteins

机译：PLiMAP:附近的标签膜相关蛋白
- 作者：Gregor P. Jose;Thomas J. Pucadyil
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
- Cross-linked;
- membrane binding;
- JournalistsMembrane ProteinsRendering;
11.Target Protein Identification on Photocatalyst-Functionalized Magnetic Affinity Beads

机译：目标蛋白质识别Photocatalyst-Functionalized磁亲和力珠子
- 作者：Michihiko Tsushima;Shinichi Sato;Keita Nakane
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
12.De Novo Protein Design Using the Blueprint Builder in Rosetta

机译：新创的蛋白质设计使用蓝图构建器在罗塞塔
- 作者：Linna An;Gyu Rie Lee
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
- Rosetta;
- Proteins;
- EngineeringConstruction Workerswire-drawing machine;
13.Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System

机译：重组蛋白的表达的方法用毕赤酵母属pastoris无细胞系统
- 作者：Rochelle Aw;Alex J. Spice;Karen M. Polizzi
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
14.Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans

机译：组蛋白结合高分辨率的净化质谱检测组蛋白转录后修饰和组蛋白变异在秀丽隐杆线虫
- 作者：Lluis Millan-Arino;Zuo-Fei Yuan;Marlies E. Oomen
- 期刊名称：《Current Protocols in Protein Science》 | 2020年第1期
- Aging;
- Caenorhabditis elegans;
- HistonesEpigeneticsPost-Translational Protein Processing;
15.De Novo Protein Design Using the Blueprint Builder in Rosetta

机译：De Novo Protein Design Using the Blueprint Builder in Rosetta
- 作者：An L.;Lee G.R.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- blueprint;
- BluePrintBDR;
- de novo protein;
- protein design;
- Rosetta;
16.Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System

机译：Methods for Expression of Recombinant Proteins Using a Pichia pastoris Cell-Free System
- 作者：Aw R.;Spice A.J.;Polizzi K.M.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
17.Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans

机译：Histone Purification Combined with High-Resolution Mass Spectrometry to Examine Histone Post-Translational Modifications and Histone Variants in Caenorhabditis elegans
- 作者：Millan-Arino L.;Yuan Z.-F.;Oomen M.E.Brandenburg S.Chernobrovkin A.Salignon J.Korner L.Zubarev R.A.Garcia B.A.Riedel C.G.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- aging;
- Caenorhabditis elegans;
- epigenetics;
- histone post-translational modifications;
- histone variants;
- mass spectrometry;
18.A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins

机译：A Rapid and Facile Purification Method for Glycan-Binding Proteins and Glycoproteins
- 作者：Welch C.J.;Kadav P.D.;Edwards J.L.Krycia J.Talaga M.L.Bandyopadhyay P.Dam T.K.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- affinity chromatography;
- agglutinin;
- galectin-3;
- glycoprotein purification;
- glycoproteomics;
- lectin;
- method development;
- non-covalent cross-linking;
- protein purification;
- proteomics;
19.Synthesis of Recombinant Human Hemoglobin With NH2-Terminal Acetylation in Escherichia coli

机译：Synthesis of Recombinant Human Hemoglobin With NH2-Terminal Acetylation in Escherichia coli
- 作者：Natarajan C.;Signore A.V.;Kumar V.Storz J.F.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- blood substitute;
- NH2-terminal acetylation;
- post-translational modification;
- recombinant hemoglobin;
20.Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations

机译：Preparation of Recombinant Membrane Proteins from Pichia pastoris for Molecular Investigations
- 作者：Guyot L.;Hartmann L.;Mohammed-Bouteben S.Caro L.Wagner R.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- detergent;
- extraction;
- integral membrane proteins;
- lipid particles reconstitution;
- Pichia pastoris;
- protoplast;
- purification;
- recombinant expression;
21.Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis

机译：Assessment of the Higher-Order Structure of Formulated Monoclonal Antibody Therapeutics by 2D Methyl Correlated NMR and Principal Component Analysis
- 作者：Arbogast L.W.;Delaglio F.;Brinson R.G.Marino J.P.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- biotherapeutics;
- methyl correlated;
- monoclonal antibodies;
- nuclear magnetic resonance;
- principal component analysis;
- SIERRA;
22.Synthesis of Recombinant Human Hemoglobin With NH2-Terminal Acetylation in Escherichia coli

机译：Synthesis of Recombinant Human Hemoglobin With NH2-Terminal Acetylation in Escherichia coli
- 作者：Natarajan C.;Signore A.V.;Kumar V.Storz J.F.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- blood substitute;
- NH2-terminal acetylation;
- post-translational modification;
- recombinant hemoglobin;
23.GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip

机译：GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip
- 作者：Shimazaki H.;Ono A.;Tsuruga M.Ueki A.Koseki-Kuno S.Toyoda T.Saito K.Sawakami K.Kariya M.Segawa O.Nakamura K.Koizuka M.Kuno A.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- automation;
- BIST;
- glycan profiling;
- lectin bead array;
24.PLiMAP: Proximity-Based Labeling of Membrane-Associated Proteins

机译：PLiMAP: Proximity-Based Labeling of Membrane-Associated Proteins
- 作者：Jose G.P.;Pucadyil T.J.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- bifunctional lipid;
- BODIPY;
- crosslinking;
- diazirine;
- fluorescence;
25.Target Protein Identification on Photocatalyst-Functionalized Magnetic Affinity Beads

机译：Target Protein Identification on Photocatalyst-Functionalized Magnetic Affinity Beads
- 作者：Tsushima M.;Sato S.;Nakane K.Nakamura H.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- affinity chromatography;
- lectin;
- magnetic beads;
- protein chemical labeling;
- proximity labeling;
- target identification;
26.Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft

机译：Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft
- 作者：Meizhe Wang;Asif Shajahan;Lauren E. PepiParastoo AzadiJoseph Zaia
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第3期
- bioinformatics;
- glycomics;
- glycoproteomics;
- GlycReSoft;
- mass spectrometry;
27.Lectin Blotting of Cell Lysates

机译：Lectin Blotting of Cell Lysates
- 作者：Tomoko Watanabe;Hiroaki Tateno
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第5期
- cell lysate;
- glycan;
- lectin;
- lectin blotting;
- protein extraction;
- protein quantification;
28.Single-Cell Glycan and RNA Sequencing (scGR-seq)

机译：Single-Cell Glycan and RNA Sequencing (scGR-seq)
- 作者：Haruki Odaka;Hiroaki Tateno
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第5期
- glycan;
- lectin;
- RNA;
- sequencing;
- single-cell;
29.Overview of Methods for Purification and Characterization of Metalloproteins

机译：Overview of Methods for Purification and Characterization of Metalloproteins
- 作者：Kevin A. Harnden;Anindya Roy;Parisa Hosseinzadeh
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第8期
- bioinorganic chemistry;
- bioinorganic spectroscopy;
- redox-active metalloproteins;
30.Methods for Enhanced Fluorescence Detection of Proteins by using Entrapped Gold Nanoparticles in Membranes

机译：Methods for Enhanced Fluorescence Detection of Proteins by using Entrapped Gold Nanoparticles in Membranes
- 作者：Nikan Momenbeitollahi;Joshua van der Zalm;Aicheng ChenHuiyan Li
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第3期
- fluorescence enhancement;
- gold nanoparticles;
- improved sensitivity;
- nitrocellulose membrane;
- protein assay;
31.Cost-effective Purification of Membrane Proteins using a Dual-detergent Strategy

机译：Cost-effective Purification of Membrane Proteins using a Dual-detergent Strategy
- 作者：Rupasree Brahma;H. Raghuraman
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第6期
32.NanoTag Nanobody Tools for Drosophila In Vitro and In Vivo Studies

机译：NanoTag Nanobody Tools for Drosophila In Vitro and In Vivo Studies
- 作者：Ah-Ram Kim;Jun Xu;Ross ChelohaStephanie E. MohrJonathan ZirinHidde L. PloeghNorbert Perrimon
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第12期
- CRISPR;
- Drosophila;
- knock-in;
- nanobody;
- NanoTag;
33.High-Throughput Analyses of Glycans, Glycosites, and Intact Glycopeptides Using C4-and C18/MAX-Tips and Liquid Handling System

机译：High-Throughput Analyses of Glycans, Glycosites, and Intact Glycopeptides Using C4-and C18/MAX-Tips and Liquid Handling System
- 作者：Shao-Yung Chen;David J. Clark;Hui Zhang
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第7期
- glycoproteomics;
- high-throughput;
- mass spectrometry;
- sample processing;
34.Fluorescence Intensity Fluctuation Analysis of Protein Oligomerization in Cell Membranes

机译：Fluorescence Intensity Fluctuation Analysis of Protein Oligomerization in Cell Membranes
- 作者：Tom D. Killeen;Sadia Rahman;Dammar N. BaduGabriel BienerMichael R. StonemanValeric? Raicu
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第3期
35.Immuno-Spin Trapping Method for the Analysis of S-Nitrosylated Proteins

机译：Immuno-Spin Trapping Method for the Analysis of S-Nitrosylated Proteins
- 作者：Esha Sircar;Detcho A. Stoyanovsky;Timothy R. BilliarRajib Sengupta
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第9期
- DMPO;
- immune-spin trapping;
- S-nitrosylation;
- thiyl radical;
- thiols;
36.Electron-Activated Tandem Mass Spectrometry Analysis of Glycosaminoglycans

机译：Electron-Activated Tandem Mass Spectrometry Analysis of Glycosaminoglycans
- 作者：Lauren E. Pepi;I. Jonathan Amster
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第4期
37.Magnetic Resonance Relaxometry for Determination of Protein Concentration and Aggregation

机译：Magnetic Resonance Relaxometry for Determination of Protein Concentration and Aggregation
- 作者：Taraban M.B.;Briggs K.T.;Yu Y.B.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
38.Determining the Effects of Neddylation on Cullin-RING Ligase-Dependent Protein Ubiquitination

机译：Determining the Effects of Neddylation on Cullin-RING Ligase-Dependent Protein Ubiquitination
- 作者：Kankan Wang;Xing Liu
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第3期
- cullin-RING ligases;
- in vitro assay;
- neddylation;
- protein purification;
- ubiquitination;
39.Protein Conjugation via SpyStapler-Mediated SpyTag/BDTag Coupling

机译：Protein Conjugation via SpyStapler-Mediated SpyTag/BDTag Coupling
- 作者：Xiao-Di Da;Xia-Ling Wu;Yajie LiuWen-Bin Zhang
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第4期
- bioconjugation;
- protein catenane;
- SpyCatcher;
- SpyStapler;
- SpyTag;
40.Purification of Ciliary Tubulin from Chlamydomonas reinhardtii

机译：Purification of Ciliary Tubulin from Chlamydomonas reinhardtii
- 作者：Orbach R.;Howard J.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- axoneme;
- cilia;
- flagella;
- microtubules;
- post-translational modifications;
- tubulin;
41.Analysis of Lysine Acetylation and Acetylation-like Acylation In Vitro and In Vivo

机译：Analysis of Lysine Acetylation and Acetylation-like Acylation In Vitro and In Vivo
- 作者：Kezhi Yan;Negar Mousavi;Xiang-Jiao Yang
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第5期
- acetyltransferase;
- acylation;
- acyltransferase;
- butyrylation;
- crotonylation;
- lactylation;
- lysine acetylation;
- propionylation;
- succinylation;
42.Methods to Detect Small Molecule Inhibition of RING E3 Ligase Activity

机译：Methods to Detect Small Molecule Inhibition of RING E3 Ligase Activity
- 作者：Ina Rothenaigner;Jara Kerstin Brenke;Kenji SchorppKamyar Hadian
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第4期
- alpha screen;
- RING E3 ligase;
- small molecules;
- TRAF6;
- Ubc13;
- ubiquitination;
43.Software to Visualize Proteins and Perform Structural Alignments

机译：Software to Visualize Proteins and Perform Structural Alignments
- 作者：Robert D. Barber
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第11期
- allostery;
- evolution;
- protein;
- quaternary;
- structure;
44.Methods for Recombinant Production and Purification of Peptides as SUMO-Peptide-Intein Fusion Proteins to Protect from Degradation

机译：Methods for Recombinant Production and Purification of Peptides as SUMO-Peptide-Intein Fusion Proteins to Protect from Degradation
- 作者：Tess Lamer;Marco J. van Belkum;John C. Vederas
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第10期
- heterologous expression;
- intein;
- peptide;
- protein purification;
- SUMO;
45.VLP-factory? and ADDomer~?: Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display

机译：VLP-factory? and ADDomer~?: Self-assembling Virus-Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display
- 作者：Duygu Sari-Ak;Joshua Bufton;Kapil GuptaFrederic GarzoniDaniel FitzgeraldChristiane SchaffitzelImre Berger
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第3期
46.Determining ADCC Activity of Antibody-Based Therapeutic Molecules using Two Bioluminescent Reporter-Based Bioassays

机译：Determining ADCC Activity of Antibody-Based Therapeutic Molecules using Two Bioluminescent Reporter-Based Bioassays
- 作者：Denise Garvin;Pete Stecha;Julia GildenJun WangJamison GrailerJim HartnettFrank FanMei CongZhijie Jey Cheng
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第11期
- ADCC;
- ADCC reporter bioassay;
- Fc function;
- HiBiT target cells;
- primary PBMC;
47.Computational Methods for Molecular Understanding of the AntibioticAminoacyl tRNA Synthetase Interaction

机译：Computational Methods for Molecular Understanding of the AntibioticAminoacyl tRNA Synthetase Interaction
- 作者：Shilpi Chowdhury;Poulami Ghosh;Nilashis Nandi
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第3期
- aminoacyl tRNA synthetase;
- antibiotic;
- interaction;
- quantum mechanical calculations;
- simulation;
48.Production and Purification of Shark and Camel Single-Domain Antibodies from Bacterial and Mammalian Cell Expression Systems

机译：Production and Purification of Shark and Camel Single-Domain Antibodies from Bacterial and Mammalian Cell Expression Systems
- 作者：Zhijian Duan;Jesse Buffington;Jessica HongMitchell Ho
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第6期
- camel VhH;
- nanobody;
- polymyxin B;
- shark Vnar;
- signal peptide;
- single-domain antibody;
49.Determining the Conformational Stability of a Protein from Urea and Thermal Unfolding Curves

机译：Determining the Conformational Stability of a Protein from Urea and Thermal Unfolding Curves
- 作者：Richard L. Thurlkill;Saul R. Trevino;J. Martin ScholtzGerald R. Grimsley
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第3期
- conformational stability;
- m-value;
- protein folding;
- thermal denaturation;
- urea denaturation;
50.Isolating and Analyzing Protein Containing Granules from Cells

机译：Isolating and Analyzing Protein Containing Granules from Cells
- 作者：Rachel A. Victor;Valery F. Thompson;Jacob C. Schwartz
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第3期
51.Two-Step Preparation of Highly Pure, Soluble HIV Protease from Inclusion Bodies Recombinantly Expressed in Escherichia coli

机译：Two-Step Preparation of Highly Pure, Soluble HIV Protease from Inclusion Bodies Recombinantly Expressed in Escherichia coli
- 作者：Sherry D.;Worth R.;Sayed Y.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- HIV protease;
- inclusion bodies;
- ion-exchange chromatography;
- protein purification;
- protein refolding;
52.Tissue N-Glycan Analysis Using LC-MS, MS/MS, and MS~n

机译：Tissue N-Glycan Analysis Using LC-MS, MS/MS, and MS~n
- 作者：Noriko Suzuki
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第7期
- 2-aminopyridine;
- N-glycomics;
- permethylation;
- reversed-phase liquid chromatography;
- SALSA;
53.Measurement of Homocitrulline, A Carbamylation-derived Product, in Serum and Tissues by LC-MS/MS

机译：Measurement of Homocitrulline, A Carbamylation-derived Product, in Serum and Tissues by LC-MS/MS
- 作者：Stéphane Jaisson;Aurore Desmons;Manon DouéLa?titia GorisseChristine PietrementPhilippe Gillery
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第4期
- carbamylation;
- homocitrulline;
- LC-MS/MS;
- protein molecular aging;
54.Target Protein Identification on Photocatalyst-Functionalized Magnetic Affinity Beads

机译：Target Protein Identification on Photocatalyst-Functionalized Magnetic Affinity Beads
- 作者：Tsushima M.;Sato S.;Nakane K.Nakamura H.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- affinity chromatography;
- lectin;
- magnetic beads;
- protein chemical labeling;
- proximity labeling;
- target identification;
55.Introduction to the Use of Linear and Nonlinear Regression Analysis in Quantitative Biological Assays

机译：Introduction to the Use of Linear and Nonlinear Regression Analysis in Quantitative Biological Assays
- 作者：Stephen W. Jarantow;Eileen D. Pisors;Mark L. Chiu
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第6期
56.In Vivo Assessment of Ferroptosis and Ferroptotic Stress in Mice

机译：In Vivo Assessment of Ferroptosis and Ferroptotic Stress in Mice
- 作者：Kana Ide;Tomokazu Souma
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第4期
- 4-HNE;
- 4-hydroxynonenal;
- ferroptosis;
- liproxstatin-1;
- mouse disease models;
57.Forward Genetic Screens of Human Glycosylation Pathways Using the GlycoGene CRISPR Library

机译：Forward Genetic Screens of Human Glycosylation Pathways Using the GlycoGene CRISPR Library
- 作者：Anju Kelkar;Theodore Groth;Sriram Neelamegham
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第4期
- CRISPR-Cas9;
- glycogene;
- glycosylation;
- lectin;
- pathway analysis;
58.Rheo-NMR Spectroscopy for Cryogenic-Probe-Equipped NMR Instruments to Monitor Protein Aggregation

机译：Rheo-NMR Spectroscopy for Cryogenic-Probe-Equipped NMR Instruments to Monitor Protein Aggregation
- 作者：Daichi Morimoto;Erik Walinda;Akihiko YamamotoUlrich SchelerKenji Sugase
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第12期
- biomechanical shear;
- Couette flow;
- protein aggregation;
- protein oligomerization;
- Rheo-NMR;
59.Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor

机译：Label-Free Protein Glycosylation Analysis Using NanoMonitor-An Ultrasensitive Electrochemical Biosensor
- 作者：Antra Ganguly;Kai-Chun Lin;Sriram MuthukumarVinay J. NagarajShalini Prasad
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第6期
60.Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine

机译：Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine
- 作者：Kamau Fahie;Bhargavi Narayanan;Fiddia ZahraRussell ReevesSteve M. FernandesGerald W. HartNatasha E. Zachara
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第5期
- analysis;
- detection;
- galactosyltransferase;
- glycosylation;
- O-GlcNAc;
- O-linked;
- signal transduction;
61.A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time

机译：A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time
- 作者：Tripathi V.;Das R.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- anisotropy;
- fluorescence spectroscopy;
- real-time;
- SUMOylation;
- viral protein;
62.A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time

机译：A Fluorescence-Based Assay to Monitor SUMOylation in Real-Time
- 作者：Tripathi V.;Das R.
- 期刊名称：《current protocols in protein science》 | 2020年第1期
- anisotropy;
- fluorescence spectroscopy;
- real-time;
- SUMOylation;
- viral protein;
63.Dot-Blotting: A Quick Method for Expression Analysis of Recombinant Proteins

机译：Dot-Blotting: A Quick Method for Expression Analysis of Recombinant Proteins
- 作者：Vibhor Mishra
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第9期
- dot-blot;
- nitrocellulose;
- protein expression;
- purification;
- recombinant protein;
- western blotting;
64.Humanization of the Shark V_(NAR)SingleDomain Antibody Using CDR Grafting

机译：Humanization of the Shark V_(NAR)SingleDomain Antibody Using CDR Grafting
- 作者：Yi-Fan Zhang;Yaping Sun;Jessica Hong
- 期刊名称：《Current Protocols in Protein Science》 | 2023年第1期
65.The Characterization of Polysorbate

机译：The Characterization of Polysorbate
- 作者：Sisi Zhang;Hui Xiao;Ning Li
- 期刊名称：《Current Protocols in Protein Science》 | 2022年第7期
- 2DLC-CAD/MS;
- forced degradation;
- lipase/esterase;
- polysorbate degradation profiling;
- polysorbate degradation;
66.Practical Methods for Expression of Recombinant Protein in the Pichia pastoris System

机译：Practical Methods for Expression of Recombinant Protein in the Pichia pastoris System
- 作者：Roghayeh Mohammadzadeh;Mohsen Karbalaei;Saman SoleimanpourArman MosavatSeyed Abdolrahim RezaeeKiarash GhazviniHadi Farsiani
- 期刊名称：《Current Protocols in Protein Science》 | 2021年第6期
- expression;
- Pichia pastoris system;
- practical methods;
- recombinant protein;
- recombinant protein expression;

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