一个水稻半矮化和花发育异常突变体的遗传分析和分子定位

摘要

从粳稻品种‘日本晴,经60 Co诱变的M2代材料中发现一个半矮化并且花发育异常突变体sd-df3,其表现为植株半矮化,分蘖增加,半包茎穗,雄蕊发育不良,无花粉.遗传分析显示,该突变体表型受1对隐性核基因控制.以杂合型突变体为母本,与广亲和品种Dular杂交,构建F2分离群体,将该基因定位在水稻第3号染色体,In/Del标记333591与333818之间的物理距离约为227 kb的范围,目前该范围内没有矮化相关基因报道.%A rice semi-dwarf and deformed flower mutant,temporarily named as sd-df3,was identified from 60Co γ-ray radiation mutation in japonica rice variety Nipponbare.The mutant was characterized by semi-dwarf,more tillers,semi-enclosed panicle,stamens dysplasia and non-pollen.Genetics analysis indicated that the mutant was controlled by a single recessive gene.The F2 mapping population was derived from a cross between the heterozygous sd-df3 genotype and a wide compatible variety Dular.The gene was located in a 227 kb region between the In/Del marker 333591 and 333818 on chromosome 3 in rice,which was assumed to be a new gene controlling semi-dwarf and deformed flower.