植物中CRISPR/Cas系统的应用研究进展

摘要

CRISPR (clustered regularly interspaced short palindromic repeat)is a new genomic editing tech-nology in recent years,which is concerned for easier manipulation,higher targeting efficiency and wider range of applications.However,the research reports about the application of CRISPR/Cas system in bac-teria and animals are more than in plants.In this review,the principle,structure,working mechanism and the progress of CRISPR/Cas system in plants were introduced in order to provide foundation to increase crop yield and cultivate resistant breeds by CRISPR/Cas system.%成簇规律间隔短回文重复—CRISPR(clustered regularly interspaced short palindromic repeat)是近年来新兴的基因组编辑技术,因其操作简便、靶向效率高和应用范围广而备受关注.国内外关于CRISPR/Cas系统在细菌和动物研究中的应用报道较多,但对其在植物研究中的应用报道较少.该文综述了CRISPR/Cas系统的发现、结构、作用机制及其在植物研究中的应用,以期为CRISPR/Cas系统在提高农作物产量及培育抗性品种等方面的应用研究奠定基础.