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Effect of increased load of high-strength food wastewater in thermophilic and mesophilic anaerobic co-digestion of waste activated sludge on bacterial community structure

机译:废水中活性污泥嗜温和中温厌氧消化中高强度食品废水负荷增加对细菌群落结构的影响

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In recent years, anaerobic co-digestion (AcoD) has been widely used to improve reactor performance, especially methane production. In this study, we applied two different operating temperatures (thermophilic and mesophilic) and gradually increased the load of food wastewater (FWW) to investigate the bacterial communities during the AcoD of waste activated sludge (WAS) and FWW. As the load of FWW was increased, methane production rate (MPR; L CH4/Ld) and methane content (%) in both Thermophilic AcoD (TAcoD) and Mesophilic AcoD (MAcoD) increased significantly; the highest MPR and methane content in TAcoD (1.423 L CH4/L d and 68.24%) and MAcoD (1.233 L CH4/L d and 65.21%) were observed when the FWW mixing ratio was 75%. However, MPR and methane yield in both reactors decreased markedly and methane production in TAcoD ceased completely when only FWW was fed into the reactor, resulting from acidification of the reactor caused by accumulation of organic acids. Pyrosequencing analysis revealed a decrease in bacterial diversity in TAcoD and a markedly different composition of bacterial communities between TAcoD and MAcoD with an increase in FWW load. For example, Bacterial members belonging to two genera Petrotoga (assigned to phylum Thermotogae) and Petrimonas (assigned to phylum Bacteroidetes) became dominant in TAcoD and MAcoD with an increase in FWW load, respectively. In addition, quantitative real-time PCR (qPCR) results showed higher bacterial and archaeal populations (expressed as 16S rRNA gene concentration) in TAcoD than MAcoD with an increase in FWW load and showed maximum population when the FWW mixing ratio was 75% in both reactors. Collectively, this study demonstrated the dynamics of key bacterial communities in TAcoD and MAcoD, which were highly affected by the load of FWW. (C) 2016 Published by Elsevier Ltd.
机译:近年来,厌氧共消化(AcoD)已被广泛用于改善反应器性能,特别是甲烷的生产。在这项研究中,我们应用了两种不同的工作温度(嗜温和中温),并逐渐增加了食品废水(FWW)的负荷,以研究废活性污泥(WAS)和FWW的AcoD期间的细菌群落。随着FWW负荷的增加,嗜热AcoD(TAcoD)和嗜温AcoD(MAcoD)的甲烷生产率(MPR; L CH4 / Ld)和甲烷含量(%)显着增加;当FWW混合比为75%时,在TAcoD(1.423 L CH4 / L d和68.24%)和MAcoD(1.233 L CH4 / L d和65.21%)中观察到最高的MPR和甲烷含量。然而,当仅将FWW进料到反应器中时,这两个反应器中的MPR和甲烷产率均显着下降,TAcoD中的甲烷生产完全停止,这是由于有机酸积聚引起的反应器酸化。焦磷酸测序分析显示,随着FWW负荷的增加,TAcoD中细菌多样性的降低以及TAcoD和MAcoD之间细菌群落的组成明显不同。例如,随着FWW负荷的增加,属于Petrotoga(属于Thermotogae属)和Petrimonas(属于Bacteroidetes属)两个属的细菌成员分别在TAcoD和MAcoD中占主导地位。此外,定量实时PCR(qPCR)结果显示,TAcoD中细菌和古细菌种群(表示为16S rRNA基因浓度)高于MAcoD,且FWW负荷增加,当FWW混合比均为75%时显示最大种群反应堆。总的来说,这项研究证明了TAcoD和MAcoD中关键细菌群落的动态,这受FWW负荷的影响很大。 (C)2016由Elsevier Ltd.出版

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