High efficiency somatic embryogensis and plant germination in grapevine cultivars Chardonnay and Brachet to a grappolo lungo

摘要

a highly efficient, reproducible method for somatic embryogenesis induction, plant recovery and embryogenic culture preservation has been developed for cvs Chardonnay and Brachet to a grappolo lungo (Vitis vinifera), starting from immature anthers and ovaries. Embryogenic induc- tion efficiency was 2/100 and 17/100 in anthers for Char- donnay and Brachetto g. L, respectively, and 14/100 in ova- ries for both cultivars.