Influence of pre-bloom sprays of benzyladenine on in vitro recovery of hybrid embryos from crosses of Thompson Seedless and 8 seeded varieties of grape (Vitis spp.)

摘要

The present investigations are part of a study to introgress downy mildew resistance in Thompson Seedless through breeding and in ovulo embryo rescue methods. The influence of sprays of benzyladenine (BA) at pre-bloom and bloom on the percentage of embryo recovery, germination and development of hybrid plants is described. BA gave mixed results among the 8 cross combinations. With some crosses, BA drastically improved these parameters. In almost all crosses, germination increased significantly with BA sprays. The influence on plant development and recovery depended on BA treatment and the male parent.