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Grapevine DNA polymorphisms revealed by microsatellite-derived marker from soybean and rice

机译:大豆和水稻中微卫星衍生标记揭示的葡萄DNA多态性

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摘要

We report detection of DNA polymorphisms in grapevine by the use of microsatellite-flanking primer pairs from soybean and rice. These "cross species" microsatellite-derived markers were checked for their inheritance patterns in controlled grapevine crosses. They produced multiple bands that segregated and can be scored as individual genetic markers of dominant type. Employed in genetic mapping studies they offer advantages such as improved reproducibility in comparison to commonly used multi-locus marker systems like RAPDs and AFLPs.
机译:我们报告通过使用大豆和水稻中的微卫星侧翼引物对检测葡萄藤中的DNA多态性。检查这些“跨物种”微卫星衍生标记在可控葡萄杂交中的遗传模式。他们产生了多个分离的条带,可以作为显性类型的单个遗传标记进行评分。与常规使用的多位点标记系统(如RAPD和AFLP)相比,它们在遗传作图研究中提供了优势,例如提高了可重复性。

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