Microplate-array diagonal-gel electrophoresis (MADGE) and melt-MADGE: tools for molecular-genetic epidemiology

摘要

Microplate-array diagonal-gel electrophoresis (MADGE) was invented for molecular-genetic epidemological studies. It com- bines direct compatibility with microplates, convenient polyacrylamide-gel electrophoresis and economy of time and regents at minimal capital cost, and enables on user to run up to several-thousand gel lanes per day for the direct assay of single- base variations. Melt-MADGE adds temporal-thermal-ramp apparatus to achieve similar throughput for de novo mutation scanning.