The impact of two-hybrid and related methods on biotechnology

摘要

Two-hybrid technology has contributed significantly to the unraveling of molecular regulatory networks by facilitating the dis- covery of protein interactions. Outgrowths of these methods are developing rapidly, including interaction mating to identify false positives and map protein networks, two-bait systems, systems not based on transcription, and systems permitting the selection of peptide aptamers to manipulate gene and allele function. These advances promise to have a significant impact on industrial biotechnology and drug development.