Protease inhibitor MG132 in cloning: no end to the nightmare

摘要

Nuclear reprogramming directed by the ooplasm is essential for producing cloned animals by somatic cell nuclear transfer (SCNT). One component of the reprogramming process is the removal of somatic histone H1 variants followed by replacement by the oocyte-specific form, H1FOO [1]. Despite occurrence of this transition in 100% of SCNT constructs, cloned embryo development remains poor and cloned embryos express numerous somatic cell characteristics [2], indicating that reprogramming is slow or incomplete.