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Impact of mercury on the activity pattern of a marker enzyme in a freshwater bivalve

机译:汞对淡水双壳类中标记酶活性模式的影响

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Sessile benthic organisms are universally accepted as bio-indicators of environmental pollution and lysosomal membrane is often the target of injury by xenobiotics resulting in destabilization. Effect of mercury on the activity pattern of the marker enzyme, Acid Phos-phatase in the gill and hepatopancreas of the freshwater mussel, Lamellidens corrianus (Lea) at 24, 72, and 168 h, post-exposure discussed. ACP activity was determined as given in Sigma Technical Bulletin (No. 104) and μmol of p-nitrophenol liberated/mg protein/h calculated. The work aimed to study the duration of exposure and concentration of the metal influence, the direction of the response of the enzyme in the two tissues studied and results show that the concentration of the metal as well as the period of exposure do influence the enzyme activity and may have predictive value as a biomarker of impending population changes. Higher concentration of the metal is assumed to have induced stress proteins like metallothioneins.
机译:底栖生物被普遍认为是环境污染的生物指标,而溶酶体膜通常是导致不稳定的异源生物伤害的目标。汞在暴露后24、72和168 h对淡水贻贝Lamellidens corrianus(Lea)的ill和肝胰腺中标志酶,酸性磷酸酶活性模式的影响。按照Sigma技术公告(第104号)中的规定确定ACP活性,并计算出释放出的μmol对硝基苯酚/ mg蛋白质/ h。这项工作旨在研究暴露的持续时间和金属浓度的影响,研究的两个组织中酶反应的方向,结果表明金属的浓度以及暴露时间确实会影响酶的活性。可能具有预测价值,可以作为即将发生的人口变化的生物标记。假定较高浓度的金属具有诱导的应激蛋白,如金属硫蛋白。

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