Peptide-imprinted polymer microspheres prepared by precipitationpolymerization using a single bi-functional monomer

摘要

A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used innprecipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres.nHighly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leuenkephalinnand Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymernmicrospheres that are able to recognize target peptides through the consensus C-terminal sequence. Theninteresting molecular binding results suggest a new approach for peptide analysis combining in situnchemical modification with MIP recognition under non-aqueous conditions.