Living cell-based allergen sensing using a high resolution two-dimensional surface plasmon resonance imager

摘要

Recently, several papers indicated that the surface plasmon resonance (SPR) technique was available tonmonitor stimulation responses of mammalian cells adhered on sensor chips. On the other hand, thennewly developed two-dimensional SPR (2D-SPR) imager system can obtain 2D-images of localnrefractive index change on the surface of a gold thin film. From these backgrounds, we expected that then2D-SPR imager can visualize the individual response of many mammalian cells, simultaneously. Here,nwe report the observation of an allergenic response of a model mast cell, rat basophilic leukaemia celln(RBL-2H3), by using the high magnification 2D-SPR imaging system after pre-sensitization with 0.1 mgnmLu00011 anti-dinitrophenyl immunoglobulin E (anti-DNP IgE). The response of the cells wasnsuccessfully observed as the increment of the SPR signal (reflection intensity) upon stimulation withn0.1–1000 ng mLu00011 DNP-modified bovine serum albumin (DNP-BSA).