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Inheritance of plant regeneration from maize (Zea mays L.) shoot meristem cultures derived from germinated seeds and the identification of associated RAPD and SSR markers

机译:玉米发芽种子分生组织培养物从玉米(Zea mays L.)植物再生的遗传及相关RAPD和SSR标记的鉴定

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摘要

The inheritance of shoot regeneration through shoot-tip meristem culture derived from maize seedling was evaluated, and the markers (RAPD and SSR) associated with this regeneration character were identified both in a group of North American maize inbreds and a crossing population. A discrete distribution of percent regeneration and no. of shoots per explant was observed in the inbred group and the F2 population. The results suggested that this regenerable trait was controlled by several major genes. Five RAPD markers were identified to be relevant to percent regeneration in maize shoot-tip culture system. One RAPD marker and three SSR markers were associated with no. of shoot per explant and its relevant traits. Of them marker BC603-1600 explained 18% of the variation for no. of shoot per explant and 16% of the variation for callus size. The BC603-1600 was sequenced and assigned in linkage group 7 based on a NCBI blast search. The information provided here should benefit to determine the genetic mechanisms involved in the maize regeneration response related to shoot meristem culture pathway and benefit to select high regenerable germplasm by using marker assisted selection.
机译:评价了来自玉米幼苗的梢尖分生组织培养的芽再生遗传,并在一组北美玉米自交系和杂交种群中鉴定了与该再生特性相关的标记(RAPD和SSR)。再生百分比和数量的离散分布。在自交系和F2 群体中观察到了每个外植体的芽数。结果表明该可再生性状受几个主要基因控制。确定了五个RAPD标记与玉米茎尖培养系统中的再生百分比有关。一个RAPD标记和三个SSR标记与否相关。外植体的芽数及其相关性状。其中标记BC603-1600解释了18%的变异。每个外植体的芽数和愈伤组织大小变化的16%。根据NCBI blast搜索对BC603-1600进行测序,并将其分配给连锁组7。此处提供的信息将有助于确定与分生组织培养途径有关的玉米再生反应的遗传机制,并有助于通过使用标记辅助选择来选择高可再生种质。

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  • 来源
    《Theoretical and Applied Genetics》 |2004年第4期|681-687|共7页
  • 作者单位

    Soybean Research Institute Northeast Agricultural University;

    Biology Department Saint Mary’s University;

    Biotechnology Division Department of Agriculture University of Guelph;

    Biotechnology Division Department of Agriculture University of Guelph;

    Biotechnology Division Department of Agriculture University of Guelph;

    Biotechnology Division Department of Agriculture University of Guelph;

    Biotechnology Division Department of Agriculture University of Guelph;

    Biotechnology Division Department of Agriculture University of Guelph;

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