Use of simple sequence repeats for authentication of sugarcane hybrids generated from Yunnan Erianthus rockii

摘要

An experiment was conducted by Yunnan Academy of Agricultural Sciences in which three clones of E. rockii, F_1 2000-7, F_12000-15, F_12000-16 were crossed with ROC10, F172 and Yunnan E. arundinaceum. The use of 4 simple sequence repeat (SSR) primers, Msscir16, Msscir26, Msscir33 and Msscir36 authenticated that E. rockii F_1 2000-15 [ROC10 (Female)x E.rockii99-9(Male)]and E.rockii F_1 2000-16[F172 (Female) x E. rockii 99-4(Male)] were the true hybrids of Yunnan E.rockii. Also, the progenies obtained by crossing between E. arundinaceum as a female parent and E. rockii F_1 2000-7 and E.rockii F_1 2000-15 as a male parent showed that F_1 06-75 (Yunnan E. rockii with E. arundinaceum) was the true hybrids. The findings may be utilization in the breeding programs tailored to develop new high yielding sugarcane varieties using E. rockii genetic stocks.