Use of Sodium Hypochlorite as Media Sterilant in Sugarcane Micropropagation at Commercial Scale

摘要

Sodium hypochlorite has been successfully utilized as media sterilant (at total active chlorine concentration 0.002% in the medium) in production of sugarcane plantlets by applying apical meristem culture technique. The process has been scaled up to produce 2.5 million sugarcane plantlets per year. Use of sodium hypochlorite in place of autoclaving has resulted in reducing considerable amount of electricity cost and ultimately reducing the cost of production of sugarcane plantlets.