Spectrophotometric Determination of Aciclovir, Cefepime Hydrochloride, Etamsylate and Metoclopramide Hydrochloride Using 1,10 Phenanthroline - Fe(Ⅲ) Reagent

摘要

A simple, rapid,sensitive and accurate method for the determination of aciclovir, cefepime HCl, etamsylate and metoclopramide HCl in pure form and in pharmaceutical formulations is developed. The method is based on the formation of tris(o-phenanthroline) iron(Ⅱ) complex (Ferroin) upon the reaction of the cited drugs with iron(Ⅲ)-o- phenanthroline mixture. The ferroin complex is colorimetrically measured at λ_(max) 510 nm against a reagent blank. Optimization of the experimental conditions is described. Beer's law is obeyed in the concentration range from 0.25-30 μg ml~(-1) with molar absorprtivities (ε) ranging from 4.796 x 10~3-9.512 x 10~4 L.mol~(-1).cm~(-1) and Sandell sensitivities (S) of 2.129 x 10~(-3) - 34.5 x 10~(-3) μg cm~(-2). The developed method is applied successfully for the determination of the cited drugs in pure forms and in the corresponding pharmaceutical formulations without any interferences from common excipients.