Promotion of Met-tRNA~Met _i Binding to Ribosomes by ylF2, a Bacterial IF2 Homolog in Yeast

摘要

Delivery of the initiator methionine transfer RNA (Met-tRNA~Met _i) to the ribosome is a key step int e initiation of protein synthesis. Previous results have indicated that this step is catalyzed by the structurally dissimilar translation factors in prokaryotes and eu- karyostes-intiation factor 2 (IF2) and eukaryotic initiation factor 2 (elF2), respectively. A bacterial IF2 homolog has been identified in both eukaryotes and archaea.