Screening For Kineses

摘要

Hedgehog (Hh) ligands are regulators of developmental patterning, and overactivity of the Hh signaling pathway is associated with some human cancers. Genetic approaches in Drosophila have identified many components of the signaling pathway, but Hh signaling is still incompletely understood, particularly in vertebrates, in which the components of the pathway differ substantially from their counterparts in flies. Evangelista et al. searched for protein kinases that influenced Hh signaling by monitoring the effects of a library of siRNA molecules on the expression of a reporter gene in mammalian cells.