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摘要

The CuBiAn is a fully automated, membrane-free chemical analyzer that measures key parameters in cell culture media, including immunoglobulin G (IgG). The instrument offers high reproducibility with low operating costs in calibration and maintenance. Pipetting, sample predilution, and temperature control are all automated to provide significant assay ranges. The CuBiAn can detect IgG concentrations along with 11 different metabolites in a single sample and sequence, IgG is detected by immuno-turbidimetry, while all other molecules are quantified by photometric enzyme assays. A wide measurement range allows for nearly unlimited flexibility for new assay methods. The key parameters measured are IgG, glucose, lactate, ammonia, glutamine, glutamate, inorganic phosphate, lactate dehydrogenase, total protein, sodium, potassium, and chlorine. It requires low sample volumes and can provide 90 results per hour.