CHEMOTAXIS CELL MIGRATION

摘要

The Millicell u-Migration Assay Kit is an innovative, slide-based platform that can be used to visualize and measure the effects of chemoattractants on the migration of adherent cells in real time. The kit is ideal for studying metastatic behavior or the effects of toxins. The Boyden chamber is the most commonly used assay for studying chemotaxis. This end-point assay uses chambers separated by a microporous filter that cells migrate through towards a chemoattractant. However, movement of cells through the filter cannot be visualized. The Millicell u-Migration Assay replaces the traditional filter with microchannels, allowing a cell's migration to be directly observed and its progress to be quantitatively measured. Real-time observation of individual cells provides insight into a number of cell migration parameters, including directionality, velocity, and the migration index of tracked cells. Up to three assays can be run in parallel on each slide with approximately 100 to 200 cells per assay.