WESTERN BLOTTING TRANSFER SYSTEM

摘要

The Trans-Blot Turbo system is a rapid Western blotting instrument that allows researchers to efficiently transfer proteins from gel to blot in as little as three minutes. Conventional tank and semi-dry Western blotting protocols require time-consuming reagent preparation and setup, followed by an electrophoretic transfer that can take up to an hour or overnight to complete. Researchers using theTrans-Blot Turbo system can overcome this bottleneck by significantly reducing their transfer time to three to seven minutes, depending on protein size. The system uses prepackaged Turbo transfer packs that include paper, buffer, and membrane (nitrocellulose or PVDF), eliminating the time and hassle associated with the preparation of typical Western blots. The Trans-Blot Turbo system delivers better transfer efficiency compared with traditional methods, enabling the detection of proteins larger than 200 kD.