STAIN-FREE PRECAST GELS

摘要

With the recently launched Criterion Tris-Glycine extended (TGX) Stain-Free precast gels, researchers can achieve protein separation, gel imaging, and data analysis in 25 minutes-without the need for staining. Current protein electrophoresis staining and destaining protocols can take up to three hours to complete: handcast and precast gels can take 45-60 minutes to run and traditional Coomassie staining can add another two hours of work before analysis. In addition to its speed, Criterion TGX Stain-Free precast gels outperformed the Coomassie staining system in terms of sensitivity, reproducibility, and performance. Unlike other precast gels, which require expensive specialized buffer systems and stains, the gels use the standard Laemmli buffer system and do not require staining, which keeps running costs low. Criterion TGX Stain-Free gels are available in 4 to 15 percent and 4 to 20 percent as well as a unique formulation, Any kD, that allow the resolution of proteins across a wide range of molecular weights (10-250 kD).