Optimize Transfections Using FuGENE® HD Transfection Reagent

摘要

Optimal DNA delivery into mammalian cells depends on multiple factors. We recommend optimizing transfection conditions for all cell types to find the best balance between maximal protein expression and minimal impact on cell viability. This report addresses the variables that should be tested when first optimizing transfection. A number of factors will contribute to transfection success as well as the biological response of the trans-fected cells. We recommend careful consideration of each of the following parameters, and illustrate successful plasmid DNA transfections using FuGENE® HD Transfection Reagent.