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Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases

机译:分子拟态通过SnRK2激酶和PP2C磷酸酶调节ABA信号传导。

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摘要

Abscisic acid (ABA) is an essential hormone for plants to survive environmental stresses. At the center of the ABA signaling network is a subfamily of type 2C protein phosphatases (PP2Cs), which form exclusive interactions with ABA receptors and subfamily 2 Snfl-related kinase (SnRK2s). Here, we report a SnRK2-PP2C complex structure, which reveals marked similarity in PP2C recognition by SnRK2 and ABA receptors. In the complex, the kinase activation loop docks into the active site of PP2C, while the conserved ABA-sensing tryptophan of PP2C inserts into the kinase catalytic cleft, thus mimicking receptor-PP2C interactions. These structural results provide a simple mechanism that directly couples ABA binding to SnRK2 kinase activation and highlight a new paradigm of kinase-phosphatase regulation through mutual packing of their catalytic sites.
机译:脱落酸(ABA)是植物在环境胁迫下生存所必需的激素。 ABA信号网络的中心是2C型蛋白磷酸酶(PP2Cs)的一个亚家族,与ABA受体和2个Snf1相关激酶(SnRK2s)形成排他性相互作用。在这里,我们报告一个SnRK2-PP2C复杂的结构,它揭示了SnRK2和ABA受体在PP2C识别中的明显相似性。在该复合物中,激酶激活环停靠在PP2C的活性位点中,而保守的PP2C的ABA敏感色氨酸插入到激酶的催化裂隙中,从而模拟了受体-PP2C的相互作用。这些结构结果提供了一种简单的机制,该机制将ABA结合直接耦合到SnRK2激酶激活,并通过相互催化位点相互堆积突出了激酶磷酸酶调节的新范式。

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  • 来源
    《Science》 |2012年第6064期|p.85-88|共4页
  • 作者单位

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA,department of Obstetrics and Gynecology, National University Hospital, Yong Loo Lin School of Medicine, Na-tional University of Singapore (NUS), Singapore 119228;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA,department of Obstetrics and Gynecology, National University Hospital, Yong Loo Lin School of Medicine, Na-tional University of Singapore (NUS), Singapore 119228;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Department of Molecular Therapeutics, Translational Re-search Institute, The Scripps Research Institute, Scripps Florida,130 Scripps Way No. 2A2, Jupiter, FL 33458, USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA,department of Obstetrics and Gynecology, National University Hospital, Yong Loo Lin School of Medicine, Na-tional University of Singapore (NUS), Singapore 119228;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Department of Molecular Therapeutics, Translational Re-search Institute, The Scripps Research Institute, Scripps Florida,130 Scripps Way No. 2A2, Jupiter, FL 33458, USA;

    Depart-ment of Molecular Pharmacology and Biological Chemistry,Life Sciences Collaborative Access Team (LS-CAT), Synchrotron Research Center, Northwestern University, Argonne, IL 60439,USA;

    Department of Horticulture and Landscape Architec-ture, Purdue University, West Lafayette, IN 47907, USA;

    Center for Drug Discovery and Design, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China;

    Center for Drug Discovery and Design, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA,department of Obstetrics and Gynecology, National University Hospital, Yong Loo Lin School of Medicine, Na-tional University of Singapore (NUS), Singapore 119228;

    department of Obstetrics and Gynecology, National University Hospital, Yong Loo Lin School of Medicine, Na-tional University of Singapore (NUS), Singapore 119228;

    De-partment of Botany and Plant Sciences, University of Califor-nia at Riverside, Riverside, CA 92521, USA;

    Department of Horticulture and Landscape Architec-ture, Purdue University, West Lafayette, IN 47907, USA;

    Department of Molecular Therapeutics, Translational Re-search Institute, The Scripps Research Institute, Scripps Florida,130 Scripps Way No. 2A2, Jupiter, FL 33458, USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA;

    Laboratory of Structural Sciences, Van Andet Research In-stitute, 333 Bostwick Avenue NE, Grand Rapids, Ml 49503,USA,VARI-SIMM Center,Center for Structure and Function of Drug Targets, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203,People's Republic of China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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