DNA METHYLATION ANALYSIS

摘要

TrueMethyl brings unprecedented clarity to the analysis of DNA by providing quantitative, accurate, and repeatable single-base resolution sequencing of the modified bases hydroxymethylcytosine (5-hmC) and methylcytosine (5-mC) for the first time. Traditional bisulfite sequencing cannot discriminate between 5-hmC and 5-mC. Recent studies have shown that at some sites in the genome the leveL. of S-hmC can be comparable to the leveL. of 5-mC, emphasizing the importance of identifying these variants accurately. The high-quality and easy-to-use TrueMethyl kits utilize innovative oxida-tive bisulfite sequencing (oxBS-Seq).TrueMethyl kits can be used with a variety of common platforms including next generation sequencing systems, methylation arrays, and targeted assays. Trials of the kits conducted at leading research centers around the world have already begun to yield new insights into genome function and highlighted the advantages of TrueMethyl relative to traditionaL. approaches.