SINGLE-CELL WHOLE-GENOME AMPLIFICATION

摘要

DNA sequence analysis and genotyping of biological samples using innovative instrumentation is often limited by the small amount of sample available. The new REPLI-g Single Cell Kit is specially designed to uniformly amplify genomic DNA (gDNA) from single cells (< 1,000 cells to as little as one bacterial or tumor cell) or purified gDNA, with negligible sequence bias and maximized genome coverage. The kit uses innovative Multiple Displacement Amplification (MDA) technology to deliver high yields of DNA that is highly suited for use with, and delivers outstanding results on, new technologies such as next generation sequencing platforms. The REPLI-g Single Cell Kit can also be used for a broad range of sample types, including purified gDNA, fresh or dried blood, and fresh or frozen tissue. Innovative ultraviolet treatment eliminates any detectable trace of residual DNA in the kit components, ensuring highly reliable amplification.