Development of simultaneous nitrification-denitrification and anammox and in-situ analysis of microbial structure in a novel plug-flow membrane-aerated sludge blanket

摘要

This study proposed a novel one-stage plug-flow microaerobic sludge blanket (PMSB) with membrane aerated for treating low carbon to nitrogen (C/N) ratio munidpal sewage. The performance of simultaneous nitrification, denitrification, and anammox in the reactor was investigated. The results illustrated that the removal efficiencies of ammonium and total nitrogen (TN) were 93.2% and 87.1% with a C/N ratio of 4. High throughput sequencing revealed that aerobic bacteria, anaerobic bacteria and facultative anaerobe could co-exist at the same time in the sludge blanket. Meanwhile, a notable correlation between the oxygen concentration and the distance of the membrane module was analyzed. It was shown that the microbial community of functional bacteria developed in different aeration sites due to the oxygen concentration gradient. Microbial community structure was analyzed depending on the sludge stratification in the sludge blanket.