Thermodynamic Investigation of Interaction between Ca Cation and Negatively Charged Phospholipid Bilayers as Studied by DSC and Isothermal Titration Calorimetry

摘要

The interaction of a negatively charged vesicle composed of dimyristoylphosphatidylglycerol (DMPG) and Ca~2+ was investigated by isothermal titration calorimetry (ITC) and differential scannign calorimetry (DSC). A schematic diagram of relative enthalpy (ΔH) versus temperature (t) was constructed from two types of calorimetric enthalpies obtained by ITC and DSC and was compared for the fully neutralized vesicle (Ca~2+-binding vesicle) and the charged vesicle (CA~2+-free vesicle).