Broad host range plasmid-based gene transfer system in the cyanobacterium Gloeobacter violaceus which lacks thylakoids

摘要

Abstract Gloeobacter violaceus, a cyanobacterium lack of thylakoids, is refractory to genetic- manipulations because its ceils are enveloped by a thick gelatinous sheath and in colonial form. In this study, a large number of single cells were obtainedby repeated pumping with a .syringe with the gelatinous sheath removed. And an exogenous broad host range plasmid pKT210 was conjugatively transferred into G. violaceus. Analyses with dot-blot hybridization and restriction mapping showed that the exogenous plasniid pKT210 had been introduced into G. violaceus and stably maintained with no alteration in its structure. pKT210 extracted from G. violaceus exconjugants could be transformed into the mcr mrr - E. coli strain DH10B but not the mcr + mrr + strain DH5 alpha which suggests that a niethy-lase system may he present in G . violaceus.