Construction and characterization of a bacterial artificial chromosome library of peach

摘要

This report briefly describes the construction and characterization and characterization of a peach [prunus persica (L.) Batch] Var. Jingyu bacterial artificial chromosome (BAC) library. The variety Jingyu has many important agronomic Characters of stone fruits, and it is a main parent in Chinese peach breeding. After cloning of the high molecular weight Peach DNA into pBeloBAC 11, we obtained over 22 000 recombinant clone. The BAC library has an average insert size Of 95 kb and represents approximately 7 times peach haploid genome equivalents. After being screened with two random- Ly amplified polymorphic DNA markers, W4 and P20, which are linked to yellow flesh and nectarine genes of peach re- Spectively, ten positive clones have been detected. This library is very useful for map-based cloning of peach genes and Physical mapping of peach genome.