Species-specific identity elements of Trna~trp

摘要

through the comparisons among 91 Trna~trp sequences from prokaryotes, archea and eukaryotes, the potential species-specific identity esements of Trna ~trp are found to be located within acceptor stem, dihydrouridine (d) stem, anticodon (AC) stem and discriminator base. Mutagenesis of B. Subtilis Trna~trp to the eukaryotic consensus se- quence, in uitro transcription and enzymatic assay of Trna~trp toward different tryptophanyl-Trna synthetases (TRPRS) were employed to shed light on these species-specific identity elements and demonstrate the accurate recognition and the coevolution between Trna and TrpRS. B. Subtilis Trna~trp with its acceptor stem and discriminator base transplanted by eukaryotic counterparts exhibited diminished reactivity toward B. Subtilis enzyme but could be efficiently aminoacylated by rat liver crude enzyme.