Rapid establishment of pure lines of silver carp (Hypophthalmichthys molirix) and bighead carp (Aristichthys nobilis)

摘要

The diversity of gynogenetic, arlificiai sex reversal and natural silver carp and bighead carp is examined using randomly amplified polymorphic DNA (RAPD) method. All of the 187 bands are obtained and 19 (10. 16% ) of them are polymorphic in gynogenetic silver carp. Meanwhile 32 ( 15 . 61 %) out of 205 bands are polymorphic in control group. In gynogenetie bighead carp a totsl of 232 bands are identified and 11 (4. 74% ) out of them are polymorphic, while 25 ( 10. 37% ) out of 241 hands are polymorphic in control group. The genetic distance of four populations is calculated and it is 0. 102 and 0. 023 for gynogenetie silver carp and gynogenetic bightead Corp respectively. The values of natural silver carp and bighead carp are (0.161 and 0. 104. Fromthe UPGMA trees constructed based on genetic distance, the sex reversal individuals that match with the gynogenetie female individuals are picked out. A new breeding process of establishing a pure line is developed.