Comparative study on the effect of integrin alpha v beta 3 on secretion of matrix metalloproteinases in human normal and carcinoma trophoblasts

摘要

The invasion of cytotrophoblast cells into the maternal endometrium during embryonic implantation is very similar to the metastasis of carcinoma cells. However, the significant difference is that the former is a highly controlled process. In this report, the effects of integrin alpha v beta 3 on the secretion of matrix metalloproteinase (MMP) were compared between normal cytotrophoblast cells and chorio-carcinoma cells by RT-PCR, gelatin zymography and immunocytochemistry. The results reveal that both normal cytotrophoblast cells and choriocarcinoma cells can express integrin alpha v beta 3. The secretion of MMPs in normal cytotrophoblast cells is up-regulated by anti-alpha v beta 3 antibody, whereas, decreased in choriocarcinoma cells. It was suggested that alpha v beta 3 can modulate the expression of MMPs in trophoblasts, and this action is carried out through distinct mechanisms in normal and carcinoma cytotrophoblast cells.