Optimization of laccase production by Botryosphaeria sp. in the presence of veratryl alcohol by the response-surface method

摘要

Botryosphaeria sp. produced tow laccases (PPO-I and PPO-II) constitutively, whose titers were enhanced by veratryl alcohol. The effect of veratryl alochol and yeast extract concentration, time of cultivation and agitation speed were evaluated by factorial analysis to select variables for optimizing the production of laccases. Maximal laccase production was determined using a second-order central-composite design and analyzed by the response-surface method.