A vector method for the representation of enzymic reactions 2. Derivation of the equations for calculating the initial rates of activated and inhibited enzymic reactions

摘要

Equations for calculating the initial rates of enzyme activation (v_a) and also of the nontrivial types of enzyme inhibition (v_i) unavailable in enzyme kinetics were derived. Analysis of the equations showed that it is incorrect to apply the (1/v_i; i) coordinates to data processing of types: Ⅰ_i and Ⅱ_i~′ of biparametrically coordinated and biparametrically discoordinated enzyme inhibition, respectively. New possibilities of data analysis of enzyme activation are considered. Examples illustrating the calculation of the K_(Ⅲ_a) and K_(Ⅳ_a) constants of the catalytic (Ⅲ_a) and associative (Ⅳ_a) types of enzyme activation are presented.