Biodegradation of di-n-butyl phthalate and expression of the 3,4-phthalate dioxygenase gene in Arthrobacter sp. ZH2 strain

摘要

The Arthrobacter sp. ZH2 strain was found to efficiently degrade di-n-butyl phthalate, the optimal temperature and pH conversion conditions of which were 30 C and 9.0, respectively. The degradation of DBP was best fitted by first-order kinetic equation and the half-life was 10.l9h. ZH_2 preferentially utilized DBP rather than dimethyl phthalate (DMP) when a minimal salt medium was added with a mixture of phthalate acid esters (PAE_s) and the degradation of DMP and DBP could not induce the degradation of di-n-octyl phthalate (DOP). Partial sequences of the 3,4-phthalate dioxygenase gene were amplified from ZH_2.The expression ratio of 3,4-phthalate dioxygenase gene increased from 1-fold to 28.84-fold when the DBP concentration was increased from 0 to 500 mg per I. To our knowledge, this is the first report regarding the detection of the response of the 3,4-phthalate dioxygenase gene to different concentrations of DBP using reverse transcription quantitative PCR (RT-qPCR).