Lipase-catalyzed acylation of lily polysaccharide in ionic liquid-containing systems

摘要

A comparative study was made of immobilized Burkholderia cepacia lipase (PSL-C)-catalyzed acylation of lily polysaccharide (LP) with vinyl acetate in organic sol vents, ionic liquids (ILs) and IL-containing systems. The degree of substitution (DS) of the modified LP was used to evaluate the extent of acylation and thus enzymatic activity. In this manner, an eco-friendly solvent, 2-methyltetrahydrofuran (MeTHF), was found to be the most suitable organic reaction medium. However, compared to MeTHF, enhanced enzyme activity was observed when 1-butyl-3-methylimidazolium tetrafluorobrate([C_4MIm][BF_4]) was used as the solvent. To further enhance the DS of the modified LP product, co-solvent mixtures of [C_4MIm][BF_4] and MeTHF were investigated. Among the various MeTHF-[C_4MIm)[BF_4] systems examined, 20% (v/v) MeTHF-[C_4MIm][BF_4] produced the highest DS. In this reaction medium, the optimal water activity, reaction temperature and time were 0.33, 55 C and 18 h, respectively, producing a product DS as high as 0.67. The PSL-C enzyme exhibited a much higher stability in the IL-containing system. Additionally, PSL-C-catalyzed acylation of LP was highly regioselective, causing acylation of only C6-OH.