Oleate esters production by bridging Clostridium acetobutylicum fermentation and Candida sp. 99-125 full-cell catalysis based on gas stripping-pervaporation unit

摘要

In this work, the acetone-butanol-ethanol (ABE) fermentation was upgraded in order to improve its economy. Fed-batch fermentation was integrated with two stage gas stripping-pervaporation unit. The permeated ABE condensate was used as substrate for oleates production. Candida sp. 99-125 cells, being rich in lipase activity, were used as full-cell catalyst. Different ABE feeding strategies in the esterification process were compared. As results, 10.1%, 68.6% and 72.5% of butyl oleate were obtained in the one-step added scenario, the stepwise-added scenario, and the simultaneous fed-batch scenario, respectively. The novel hybrid process was energy-saving, environmentally friendly and profitable, showing promise in future industrialization.